{"id":905,"date":"2022-12-09T18:37:29","date_gmt":"2022-12-09T18:37:29","guid":{"rendered":"https:\/\/x-bardiagnostics.com\/?page_id=905"},"modified":"2022-12-11T14:27:55","modified_gmt":"2022-12-11T14:27:55","slug":"preanalytical-phase","status":"publish","type":"page","link":"https:\/\/x-bardiagnostics.com\/index.php\/preanalytical-phase\/","title":{"rendered":"Preanalytical Phase"},"content":{"rendered":"<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/x-bardiagnostics.com\/wp-content\/uploads\/2022\/12\/Slide11-1024x576.jpg\" alt=\"\" class=\"wp-image-935\" srcset=\"https:\/\/x-bardiagnostics.com\/wp-content\/uploads\/2022\/12\/Slide11-1024x576.jpg 1024w, https:\/\/x-bardiagnostics.com\/wp-content\/uploads\/2022\/12\/Slide11-300x169.jpg 300w, https:\/\/x-bardiagnostics.com\/wp-content\/uploads\/2022\/12\/Slide11-768x432.jpg 768w, https:\/\/x-bardiagnostics.com\/wp-content\/uploads\/2022\/12\/Slide11-1140x641.jpg 1140w, https:\/\/x-bardiagnostics.com\/wp-content\/uploads\/2022\/12\/Slide11-720x405.jpg 720w, https:\/\/x-bardiagnostics.com\/wp-content\/uploads\/2022\/12\/Slide11.jpg 1280w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n<p>Step 1:&nbsp; The sample is placed in a capillary tube Bowl which contain buffers,&nbsp;PrepGem&nbsp;extraction enzyme, and Molecular Beacons master mix ( no micro fluidics required by instrument).\u200b<\/p>\n\n\n\n<p>Step 2:&nbsp; The capillary tubes are rotated to the 95 degrees C heater plate and held for \u00bd minutes to extract DNA from the sample.\u200b<\/p>\n\n\n\n<p>Step: 3:&nbsp; The capillary tubes are rotated to the 40 degrees C heater plate where the enzyme is inactivated.&nbsp; At the same time the wax plug is melted releasing the polymerase and initiating the reaction., \u201cHot start Polymerase\u201d\u200b<\/p>\n\n\n\n<p>Step 4:&nbsp; The total solution flows into the lower stem of the capillary tube where it is cycle thru all temperatures 95 degrees C, 40 degrees C, and 60 degrees C for 20-30 cycles.\u200b<\/p>\n\n\n\n<p>Step 5:&nbsp; After the last cycle each capillary is rotated to the station located in&nbsp;&nbsp; the center section of the processing station and each capillary tube is read by the fluorimeter individually.\u200b<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Step 1:&nbsp; The sample is placed in a capillary tube Bowl which contain buffers,&nbsp;PrepGem&nbsp;extraction enzyme, and Molecular Beacons master mix ( no micro fluidics required by instrument).\u200b Step 2:&nbsp; The capillary tubes are rotated to the 95 degrees C heater plate and held for \u00bd minutes to extract DNA from the sample.\u200b Step: 3:&nbsp; The&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-905","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/pages\/905","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/comments?post=905"}],"version-history":[{"count":6,"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/pages\/905\/revisions"}],"predecessor-version":[{"id":975,"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/pages\/905\/revisions\/975"}],"wp:attachment":[{"href":"https:\/\/x-bardiagnostics.com\/index.php\/wp-json\/wp\/v2\/media?parent=905"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}