Step 1:  The sample is placed in a capillary tube Bowl which contain buffers, PrepGem extraction enzyme, and Molecular Beacons master mix ( no micro fluidics required by instrument).​

Step 2:  The capillary tubes are rotated to the 95 degrees C heater plate and held for ½ minutes to extract DNA from the sample.​

Step: 3:  The capillary tubes are rotated to the 40 degrees C heater plate where the enzyme is inactivated.  At the same time the wax plug is melted releasing the polymerase and initiating the reaction., “Hot start Polymerase”​

Step 4:  The total solution flows into the lower stem of the capillary tube where it is cycle thru all temperatures 95 degrees C, 40 degrees C, and 60 degrees C for 20-30 cycles.​

Step 5:  After the last cycle each capillary is rotated to the station located in   the center section of the processing station and each capillary tube is read by the fluorimeter individually.​